Fate Bias Inference in Lymphoid progenitors

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Identified clusters after k-medoids clustering & used sorting gates

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Compare one cluster against all the other clusters

Choose a cluster

mean.ncl = mean expression of gene not in cluster

mean.cl = mean expression of gene in cluster

fc = fold change compared to all other clusters

pv = p-value

padj = adjusted p-value

Plot gene expression in tSNE-Map

Enter a genesymbol

Compare gene expression of two clusters

Choose cluster 1

Choose cluster 2

Set p-value threshhold

Set adjusted p-value threshhold

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Choose a dimensional reduction

tSNE = t-distributed stochastic neighbor embedding

cmd = classical multidiensional scaling

lle = local linear embedding

dm = modified local linear embedding

(Loading of data takes app. 30s)

Dimensional reduction

Plot fate bias in dimensional reductions

Dimensional reduction

Target cluster

Dimensional reduction

Target cluster

Plot gene expression in dimensional reductions

Dimensional reduction

Select Gene

Use logarithmic scale (log2)

Dimensional reduction

Select Gene

Use logarithmic scale (log2)

Plot fate bias in 2 genes correlation plot

Target Cluster

Gene on x-axis

Gene on y-axis

Show cluster number

Enter cluster numbers for plotting separated by space

Target Cluster

Gene on x-axis

Gene on y-axis

Show cluster number

Enter cluster numbers for plotting separated by space

Important genes for fate bias classification

Target cluster